A Study Of Selected Environment Factors And Their Effect On The Academic Achievement Of The Pupils Attending The Rand Elementary School Kaufman, Texas

Environment is all the conditions, circumstances, and influences, surrounding and affecting the development of an organism.. The individual organism comes into being and develops in a maternal environment which supplies nourishment and other biological needs. The environment into which each of us is born supplies the material and social sustenance with which we build our lives and without which none of us could live or develop as human beings. Our environment supplies food, shelter, and clothing; the language that we learn to speak, established customs, traditions, beliefs, modes of behavior, and a growing store of human experiences. The actual environment for any individual is limited to the impressions that he receives and to which he reacts. After birth, there is a powerful impulse to grow within the child. Surrounding him are the forces of culture into which he has been born and the immediate environment in which he is to live. From the moment of his coming, this environment will influence the shaping of his habits and the molding of his character. Unaware to the child, he helps to create his environment. Through his experiences and all his ways he commands attention, makes impressions, and without so intending, influences the attitude of his elders

Electron microscopic visualization of tRNA genes with ferritin-avidin: biotin labels

A method is described for indirect electron microscopic visualization and mapping of tRNA and other short transcripts hybridized to DNA. This method depends upon the attachment of the electron-dense protein ferritin to the RNA, the binding being mediated by the remarkably strong association of the egg white protein avidin with biotin. Biotin is covalently attached to the 3' end of tRNA using an NH2 (CH2) 5NH2 bridge. The tRNA-biotin adduct is hybridized to complementcrry DNA sequences present in a single stranded nonhomology loop of a DNA:DNA heteroduplex. Avidin, covalently crosslinked to ferritin is mixed with the heteroduplex and becomes bound to the hybridized tRNA-biotin. Observation of the DNA:RNA-biotin:avidin-ferritin complex by electron microsdopy specifically and accurately reveals the position of the tRNA gene, with a frequency of labeling of approximately 50%

Dynamic capabilities : their effect on performance mediated by product integration in the highly acquisitive software industry

Building on behavioural theory employing dynamic capabilities, this paper examines how firms create competitive advantage through innovation over time after multiple mergers and acquisitions. Mergers and acquisitions are a way to acquire gaps and prominently missing features and functions; the firm then has only to assimilate them into their portfolio. This research is focused on the acquirer’s ability towards obtaining performance from product integration and is set within the context of highly acquisitive software-houses; those organisations involved in the sales and manufacture of business software products. The ability to realign and innovate this will increase performance over the long term

Gateways: Expanding knowledge through broader participation

This new journal, Gateways: International Journal of Community Research and Engagement, responds to a growing global movement of university-collaborative research initiatives. It also strives to fill a gap created by the sparse number of journals which publish outcomes of community-engaged research and work concerning community engagement. We seek articles based on research that is the result of actively engaged research-practitioner collaborative projects, has the potential of informing community-based activities or develops understanding of community engagement. Combining different knowledge bases that have traditionally been separated into academic and non-academic worlds can dramatically increase information flowing to scholars, community leaders and activists seeking to improve the quality of life in local communities around the world. We also wish to encourage work that contributes to the scholarship of engagement

Detection of Galba truncatula, Fasciola hepatica and Calicophoron daubneyi environmental DNA within water sources on pasture land, a future tool for fluke control?

Abstract Background: Increasing trematode prevalence and disease occurrence in livestock is a major concern. With the global spread of anthelmintic resistant trematodes, future control strategies must incorporate approaches focusing on avoidance of infection. The reliance of trematodes on intermediate snail hosts to successfully complete their life-cycle means livestock infections are linked to the availability of respective snail populations. By identifying intermediate snail host habitats, infection risk models may be strengthened whilst farmers may confidently apply pasture management strategies to disrupt the trematode life-cycle. However, accurately identifying and mapping these risk areas is challenging. Methods: In this study, environmental DNA (eDNA) assays were designed to reveal Galba truncatula, Fasciola hepatica and Calicophoron daubneyi presence within water sources on pasture land. eDNA was captured using a filter-based protocol, with DNA extracted using the DNeasy? PowerSoil? kit and amplified via PCR. In total, 19 potential G. truncatula habitats were analysed on four farms grazed by livestock infected with both F. hepatica and C. daubneyi. Results: Galba truncatula eDNA was identified in 10/10 habitats where the snail was detected by eye. Galba truncatula eDNA was also identified in four further habitats where the snail was not physically detected. Fasciola hepatica and C. daubneyi eDNA was also identified in 5/19 and 8/19 habitats, respectively. Conclusions: This study demonstrated that eDNA assays have the capabilities of detecting G. truncatula, F. hepatica and C. daubneyi DNA in the environment. Further assay development will be required for a field test capable of identifying and quantifying F. hepatica and C. daubneyi infection risk areas, to support future control strategies. An eDNA test would also be a powerful new tool for epidemiological investigations of parasite infections on farms.publishersversionPeer reviewe

Interactions between 2′-fluoro-(carbamoylpyridinyl)deschloroepibatidine analogues and acetylcholine-binding protein inform on potent antagonist activity against nicotinic receptors

Low-nanomolar binding constants were recorded for a series of six 2′-fluoro-(carbamoylpyridinyl)deschloroepibatidine analogues with acetylcholine-binding protein (AChBP). The crystal structures of three complexes with AChBP reveal details of molecular recognition in the orthosteric binding site and imply how the other three ligands bind. Comparisons exploiting AChBP as a surrogate for α4β2 and α7 nicotinic acetylcholine receptors (nAChRs) suggest that the key interactions are conserved. The ligands interact with the same residues as the archetypal nAChR agonist nicotine yet display greater affinity, thereby rationalizing their in vivo activity as potent antagonists of nicotine-induced antinociception. An oxyanion-binding site is formed on the periphery of the AChBP orthosteric site by Lys42, Asp94, Glu170 and Glu210. These residues are highly conserved in the human α4, β2 and α7 nAChR sequences. However, specific sequence differences are discussed that could contribute to nAChR subtype selectivity and in addition may represent a point of allosteric modulation. The ability to engage with this peripheral site may explain, in part, the function of a subset of ligands to act as agonists of α7 nAChR

Parental, prenatal, and neonatal associations with ball skills at age 8 using an exposome approach.

There is little consistency in the literature concerning factors that influence motor coordination in children. A hypothesis-free "exposome" approach was used with 7359 children using longitudinal information covering 3 generations in regard to throwing a ball accurately at age 7 years. The analyses showed an independent robust negative association with mother's unhappiness in her midchildhood (6-11 years). No such association was present for study fathers. The offspring of parents who described themselves as having poor eyesight had poorer ability. This hypothesis-free approach has identified a strong negative association with an unhappy childhood. Future studies of this cohort will be used to determine whether the mechanism is manifest through differing parenting skills, or a biological mechanism reflecting epigenetic effects